Virus:Article Title: Pyruvate is a natural suppressor of interferon signaling by inducing STAT1 protein pyruvylation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-PKM2 Cell Signaling Technology Cat.# 4053S; RRID: AB_1904096 Anti-PKM1 Cell Signaling Technology Cat.# 7067S; RRID: AB_2715534 Anti-pJAK1 Cell Signaling Technology Cat.# 3331S; RRID: AB_2265057 Anti-pTyk2 Cell Signaling Technology Cat.# 9321; RRID: AB_2303972 Anti-JAK1 Santa Cruz Cat.# sc-1677; RRID: AB_627836 Anti-Tyk2 Cell Signaling Technology Cat.# 14193; RRID: AB_2798419 Anti-Ubiquitin (Ub) Santa Cruz Cat.# 12987-1-AP; RRID: AB_628423 Anti-pY701 (STAT1) Cell Signaling Technology Cat.# 9167; RRID: AB_561284 Anti-Flag Sigma Cat.# F7425; RRID:AB_439687 Anti-STAT1 Cell Signaling Technology Cat.# 9172S; RRID: AB_2198300 Anti-STAT2 Cell Signaling Technology Cat.# 72604S; RRID: AB_2799824 Anti-Myc Abmart Cat.# M20002; RRID: AB_2861172 Anti-VSV-G abcam Cat.# ab1874; RRID: AB_302646 Anti-β-Actin Proteintech Cat.# 66009-1-Ig; RRID: AB_2687938 Anti-Tubulin Proteintech Cat.# 66031-1-Ig; RRID: AB_11042766 Anti-IFNAR2 Affinity Cat.# DF2535; RRID: AB_2839741 Anti-GAPDH Affinity Cat.# T0004; RRID: AB_2833041 Anti-STAT1-Pyru-K201 PTM BIO Inc. N/A Acylation Antibody Sampler Kit Plus PTM BIO Inc. Cat.# PTM6680; RRID: AB_3674187 Bacterial and virus strains DH5α Transgen Biotech Cat.# CD201 VSV Dr. Chen Wang from China Pharmaceutical University N/A VSV-GFP Dr. Chunsheng Dong from Soochow University, China N/A SeV Dr. Chen Wang from China Pharmaceutical University N/A Chemicals, peptides, and recombinant proteins Human IFNɑ PEPROTECH Cat.# 300-02A TRNzol Universital RNA Reagent TIANGEN Cat.# A0220A01 Mouse IFNβ R&D Systems Cat.# 8234-MB Human IFNγ R&D Systems Cat.# 285-IF-100/CF Biotin-Pyruvate Shanghai Nafu Biotechnology Co., Ltd N/A Sodium pyruvate Sigma-Aldrich Cat.# P8574 Sodium L-lactate Sigma-Aldrich Cat.# L7022 UK-5099 Selleck Cat.# 5317 D-Mannitol Selleck Cat.# S2381 2-Deoxy-D-glucose (2DG) Sigma-Aldrich Cat.# D8375 recombinant STAT1 Protein MCE HY-P73628 CDQKQEQLLLKK(pyruvoyl)MY Chinese Peptide Co Ltd N/A Critical commercial assays QuickChange Lightning site-Directed Mutagenesis Kit Stratagene Cat.# 210518 Human Lymphocyte Separation Medium Kit DAKEWE Cat.# 7111011 (Continued on next page) ll OPEN ACCESS e1 Cell 189, 1–15.e1–e5, April 2, 2026 Please cite this article in press as: Zuo et al., Pyruvate is a natural suppressor of interferon signaling by inducing STAT1 protein pyruvylation, Cell (2026), https://doi.org/10.1016/j.cell.2026.01.023 Article ..
Recombinant:Article Title: Pyruvate is a natural suppressor of interferon signaling by inducing STAT1 protein pyruvylation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-PKM2 Cell Signaling Technology Cat.# 4053S; RRID: AB_1904096 Anti-PKM1 Cell Signaling Technology Cat.# 7067S; RRID: AB_2715534 Anti-pJAK1 Cell Signaling Technology Cat.# 3331S; RRID: AB_2265057 Anti-pTyk2 Cell Signaling Technology Cat.# 9321; RRID: AB_2303972 Anti-JAK1 Santa Cruz Cat.# sc-1677; RRID: AB_627836 Anti-Tyk2 Cell Signaling Technology Cat.# 14193; RRID: AB_2798419 Anti-Ubiquitin (Ub) Santa Cruz Cat.# 12987-1-AP; RRID: AB_628423 Anti-pY701 (STAT1) Cell Signaling Technology Cat.# 9167; RRID: AB_561284 Anti-Flag Sigma Cat.# F7425; RRID:AB_439687 Anti-STAT1 Cell Signaling Technology Cat.# 9172S; RRID: AB_2198300 Anti-STAT2 Cell Signaling Technology Cat.# 72604S; RRID: AB_2799824 Anti-Myc Abmart Cat.# M20002; RRID: AB_2861172 Anti-VSV-G abcam Cat.# ab1874; RRID: AB_302646 Anti-β-Actin Proteintech Cat.# 66009-1-Ig; RRID: AB_2687938 Anti-Tubulin Proteintech Cat.# 66031-1-Ig; RRID: AB_11042766 Anti-IFNAR2 Affinity Cat.# DF2535; RRID: AB_2839741 Anti-GAPDH Affinity Cat.# T0004; RRID: AB_2833041 Anti-STAT1-Pyru-K201 PTM BIO Inc. N/A Acylation Antibody Sampler Kit Plus PTM BIO Inc. Cat.# PTM6680; RRID: AB_3674187 Bacterial and virus strains DH5α Transgen Biotech Cat.# CD201 VSV Dr. Chen Wang from China Pharmaceutical University N/A VSV-GFP Dr. Chunsheng Dong from Soochow University, China N/A SeV Dr. Chen Wang from China Pharmaceutical University N/A Chemicals, peptides, and recombinant proteins Human IFNɑ PEPROTECH Cat.# 300-02A TRNzol Universital RNA Reagent TIANGEN Cat.# A0220A01 Mouse IFNβ R&D Systems Cat.# 8234-MB Human IFNγ R&D Systems Cat.# 285-IF-100/CF Biotin-Pyruvate Shanghai Nafu Biotechnology Co., Ltd N/A Sodium pyruvate Sigma-Aldrich Cat.# P8574 Sodium L-lactate Sigma-Aldrich Cat.# L7022 UK-5099 Selleck Cat.# 5317 D-Mannitol Selleck Cat.# S2381 2-Deoxy-D-glucose (2DG) Sigma-Aldrich Cat.# D8375 recombinant STAT1 Protein MCE HY-P73628 CDQKQEQLLLKK(pyruvoyl)MY Chinese Peptide Co Ltd N/A Critical commercial assays QuickChange Lightning site-Directed Mutagenesis Kit Stratagene Cat.# 210518 Human Lymphocyte Separation Medium Kit DAKEWE Cat.# 7111011 (Continued on next page) ll OPEN ACCESS e1 Cell 189, 1–15.e1–e5, April 2, 2026 Please cite this article in press as: Zuo et al., Pyruvate is a natural suppressor of interferon signaling by inducing STAT1 protein pyruvylation, Cell (2026), https://doi.org/10.1016/j.cell.2026.01.023 Article ..
Mutagenesis:Article Title: Pyruvate is a natural suppressor of interferon signaling by inducing STAT1 protein pyruvylation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-PKM2 Cell Signaling Technology Cat.# 4053S; RRID: AB_1904096 Anti-PKM1 Cell Signaling Technology Cat.# 7067S; RRID: AB_2715534 Anti-pJAK1 Cell Signaling Technology Cat.# 3331S; RRID: AB_2265057 Anti-pTyk2 Cell Signaling Technology Cat.# 9321; RRID: AB_2303972 Anti-JAK1 Santa Cruz Cat.# sc-1677; RRID: AB_627836 Anti-Tyk2 Cell Signaling Technology Cat.# 14193; RRID: AB_2798419 Anti-Ubiquitin (Ub) Santa Cruz Cat.# 12987-1-AP; RRID: AB_628423 Anti-pY701 (STAT1) Cell Signaling Technology Cat.# 9167; RRID: AB_561284 Anti-Flag Sigma Cat.# F7425; RRID:AB_439687 Anti-STAT1 Cell Signaling Technology Cat.# 9172S; RRID: AB_2198300 Anti-STAT2 Cell Signaling Technology Cat.# 72604S; RRID: AB_2799824 Anti-Myc Abmart Cat.# M20002; RRID: AB_2861172 Anti-VSV-G abcam Cat.# ab1874; RRID: AB_302646 Anti-β-Actin Proteintech Cat.# 66009-1-Ig; RRID: AB_2687938 Anti-Tubulin Proteintech Cat.# 66031-1-Ig; RRID: AB_11042766 Anti-IFNAR2 Affinity Cat.# DF2535; RRID: AB_2839741 Anti-GAPDH Affinity Cat.# T0004; RRID: AB_2833041 Anti-STAT1-Pyru-K201 PTM BIO Inc. N/A Acylation Antibody Sampler Kit Plus PTM BIO Inc. Cat.# PTM6680; RRID: AB_3674187 Bacterial and virus strains DH5α Transgen Biotech Cat.# CD201 VSV Dr. Chen Wang from China Pharmaceutical University N/A VSV-GFP Dr. Chunsheng Dong from Soochow University, China N/A SeV Dr. Chen Wang from China Pharmaceutical University N/A Chemicals, peptides, and recombinant proteins Human IFNɑ PEPROTECH Cat.# 300-02A TRNzol Universital RNA Reagent TIANGEN Cat.# A0220A01 Mouse IFNβ R&D Systems Cat.# 8234-MB Human IFNγ R&D Systems Cat.# 285-IF-100/CF Biotin-Pyruvate Shanghai Nafu Biotechnology Co., Ltd N/A Sodium pyruvate Sigma-Aldrich Cat.# P8574 Sodium L-lactate Sigma-Aldrich Cat.# L7022 UK-5099 Selleck Cat.# 5317 D-Mannitol Selleck Cat.# S2381 2-Deoxy-D-glucose (2DG) Sigma-Aldrich Cat.# D8375 recombinant STAT1 Protein MCE HY-P73628 CDQKQEQLLLKK(pyruvoyl)MY Chinese Peptide Co Ltd N/A Critical commercial assays QuickChange Lightning site-Directed Mutagenesis Kit Stratagene Cat.# 210518 Human Lymphocyte Separation Medium Kit DAKEWE Cat.# 7111011 (Continued on next page) ll OPEN ACCESS e1 Cell 189, 1–15.e1–e5, April 2, 2026 Please cite this article in press as: Zuo et al., Pyruvate is a natural suppressor of interferon signaling by inducing STAT1 protein pyruvylation, Cell (2026), https://doi.org/10.1016/j.cell.2026.01.023 Article ..
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